Lab #3 Meiosis and Mitosis

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Lab 3 Mitosis and Meiosis
BIO201L
Student Name: Click here to enter text.
Kit Code (located on the lid of your lab kit): Click here to enter text.
Pre-Lab Questions
”1. What are chromosomes made of?”
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”2. Compare and contrast mitosis and meiosis. ”
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”3. Cancer is a disease related to uncontrolled cell division. Investigate two known causes for
these rapidly dividing cells and use this knowledge to invent a drug that would inhibit the
growth of cancer cells. ”
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Experiment 1: Observation of Mitosis in a Plant Cell
Table 1: Mitosis Predictions
Predictions:
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Supporting Evidence:
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Table 2: Mitosis Data
Number of Cells in Each
Stage
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”Interphase”
”Prophase” Click here to enter text.
Stage
”Metaphase”
”Anaphase”
”Telophase”
”Cytokinesis”
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Total Number of Cells
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Calculated % of Time Spent in
Each Stage
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Post-Lab Questions
”1. Label the arrows in the slide image below with the appropriate stage of the cell cycle. ”
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B- Click here to enter text.
C- Click here to enter text.
D- Click here to enter text.
E- Click here to enter text.
Lab 3 Mitosis and Meiosis
BIO201L
F- Click here to enter text.
”2. What stage were most of the onion root tip cells in? Does this make sense? ”
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”3. As a cell grows, what happens to its surface area : volume ratio? (Think of a balloon being
blown up). How is this changing ratio related to cell division? ”
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Lab 3 Mitosis and Meiosis
BIO201L
”4. What is the function of mitosis in a cell that is about to divide? ”
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”5. What would happen if mitosis were uncontrolled? ”
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”6. How accurate were your time predication for each stage of the cell cycle? ”
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”7. Discuss one observation that you found interesting while looking at the onion root tip cells.”
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Experiment 2: Following Chromosomal DNA Movement through Mitosis
”Prophase: ”
”Metaphase: ”
”Anaphase: ”
Lab 3 Mitosis and Meiosis
”Telophase: ”
Experiment 3: Following Chromosomal DNA Movement through Meiosis
”Trial #1: ”
Table 1: Chromatid Number
Prophase I:
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Metaphase I:
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Anaphase I:
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Telophase I:
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First Cytokinesis:
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Prophase II:
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Metaphase II:
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Anaphase II:
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BIO201L
Lab 3 Mitosis and Meiosis
”Trial #2: ”
”Prophase I: ”
”Metaphase I: ”
”Anaphase I: ”
Telophase II:
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Second Cytokinesis:
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BIO201L
Lab 3 Mitosis and Meiosis
”Telophase I: ”
”Prophase II: ”
”Metaphase II: ”
”Anaphase II: ”
BIO201L
Lab 3 Mitosis and Meiosis
BIO201L
”Telophase II: ”
Post-Lab Questions
”1. What is the state of the DNA at the end of meiosis I? What about at the end of meiosis II? ”
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”2. Why are chromosomes important? ”
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”3. How are meiosis I and meiosis II different? ”
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”4. How many chromosomes were present when meiosis I started? ”
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”5. Why is it necessary to reduce the number of chromosomes in gametes, but not in other
cells? ”
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”6. Blue whales have 44 chromosomes in every cell. Determine how many chromosomes you
would expect to find in the following: ”
”Sperm Cell: ” Click here to enter text.
”Egg Cell: ” Click here to enter text.
”Daughter Cell from Mitosis: ” Click here to enter text.
”Daughter Cell from Meiosis II: ” Click here to enter text.
Experiment 5: The Importance of Cell Cycle Control
Lab 3 Mitosis and Meiosis
BIO201L
Data:
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3. Click here to enter text.
4. Click here to enter text.
5. Click here to enter text.
Post-Lab Questions
”1. Record your hypothesis from Step 1 in the Procedure section here. ”
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”2. What do your results indicate about cell cycle control? ”
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”3. Suppose a person developed a mutation in a somatic cell which diminishes the performance
of the body’s natural cell cycle control proteins. This mutation resulted in cancer yet, but was
effectively treated with a cocktail of cancer-fighting techniques. Is it possible for this person’s
future children to inherit this cancer causing mutation? Be specific when you explain why or
why not. ”
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”4. Why do cells which lack cell cycle control exhibit karyotypes which look physically different
than cells with normal cell cycle. ”
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”5. What are HeLa cells? Why are HeLa cells appropriate for this experiment? ”
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”6. Research the function of the protein called p53. What does this function do? Explain how it
can affect cell cycle control. ”
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”7. What is the Philadelphia chromosome? How is this chromosome related to cancer? Identify
how this chromosome appears physically different on a karyotype than it appears on a
karyotype of normal chromosomes. ”
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