micro biology lab questions
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25/ Bacterial Culture Characteristics
1/ After the incubation period, why is it best to refrigerate culture plates and tubes rather than storing
them at room temperature?
2/ How can you account for the observation that a mass or colony of bacteria shows pigmentation while
the individual cells have transport cytoplasm?
3/ Describe the factors that may alter the expression of the cultural characteristics of bacteria
4/ Determine which cultural characteristics noted in this exercise are more distinctive than others
5/ Suppose your unknown prefers grow at 37C, yet both slants show similar growth. Provide some reasons
to account for this observation
26/ Biochemical characteristics of bacteria
1/ For each of the tests you preformed in this exercise, determine if the test is identifying the presence or
absence of a substrate or a product.
2/ Why is it important to use pure cultures when performing the biochemical tests on bacteria?
3/ Explain the practical significance of the production of hydrogen sulfide and indole by bacteria.
4/ How might you explain the bubbles that appear when hydrogen peroxide is applied to a wound even
though there may be no bacteria present?
5/ Explain why biochemical characteristics serve as a fingerprint for a bacterial species.
27/ The effect of antibiotics on bacteria
1/ List several factors that must be standardized to enhance the usefulness of the antibiotic susceptibility
test by the Kirby-Bauer method.
2/ Which antibiotics may be broad-spectrum antibiotics and which may be narrow-spectrum antibiotics, as
determined from the results in this exercise?
3/ How might one explain the appearance of colonies of bacteria within the zone of inhibition?
4/ Why is it more advisable to use a swab instead of a loop for preparing a lawn of bacteria on an agar
5/ Explain why a shorter incubation time of 16 to 18 hours is preferred in this test too the useful 24 to 48
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